bsl 2 checklist

Follow requirements and practices for your assigned BSL. Biosafety Level 2 - Laboratory Training Requirements.


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Microsoft Word - BSL-2 Checklistdoc Version.

. Dispose of used gloves with other contaminated laboratory waste. Comments Unless excluded pursuant to paragraph f of this section a person may not import into the United States any infectious biological agent infectious substance or vector unless. 072321 Laboratory Assistance Visit Checklist BSL2 laboratories A procedure-based approach comprising the practices personal protective equipment facilities and implementation of your laboratorys Standard Operating Procedures SOPs must be taken when evaluating safety and compliance.

Eating drinking handling contact lenses and applying cosmetics is forbidden in the laboratory. Smoking chewing gum handling contacts applying cosmetics is. What to keep in mind.

Standard Microbiological Practices Access Hand washing Food Eye protection Pipetting Sharps Splashes Aerosols Decontamination. Response Standard Microbiological Practices Reference Statement. 11618 Laboratory Assistance Visit Checklist BSL2 laboratories A procedure-based approach comprising the practices personal protective equipment facilities and implementation of your laboratorys Standard Operating Procedures SOPs must be taken when evaluating safety and compliance.

Personnel must have specific training in the procedures conducted in the BSL-2 cleanroom or laboratory. Instructions Using the checklist below inspect your lab and note any deficiencies that need to be addressed the PI may assign a senior lab member to complete the checklist but the PI must reviews date and sign the checklist. They are based on the recommendation of the Biosafety in Microbiological and Biomedical Laboratories BMBL 5th Edition 2007.

Make sure to review the checklist as advertised. BSL-1 is designated for those working with microbes that don. BSL-2 Commissioning Check-list Revision date.

April 2021 Revision 2 421 BSL-2 Commissioning Checklist Page 2 of 2 contaminated materials if they are removed from the laboratory for autoclaving. Biosafety Level 2 builds upon BSL-1. For assistance refer to the Checklist for New BSL-2 Personnel.

The following are procedures are used with BSL-2 containment requirements. Biosafety Level 2 Biosafety Level 2 BSL-2 builds upon BSL-1. The purpose of the four levels is to distinguish between different types of hazards and to provide protective measures for each level as each level has different safety requirements.

Are there written procedures for responding to exposure incidents. Experiment areas of lesser biohazard potential are carefully demarcated. Click find out more to.

Pathogenic or infections organisms that pose a moderate health hazard. Laboratory Biosafety Compliance Inspection Checklist Biosafety Level 2 Section of the Biological Safety in Microbiological Biomedical and Laboratories 5thedition. BSL-2 is suitable for work involving agents that pose moderate hazards to personnel and the environment.

ALL biological materials ie. BMBLC4-c In addition BSL-2 laboratory workers should. 1110 Yes No 12.

We are a privately funded 501c3 not-for-profit corporation and are proud to boast more than 2 million supporters across the country. Yes no NA No food or drinks consumed or stored in the lab. BSL2 differs from BSL- -.

Examples of agents typically worked with in a BSL-2 include equine encephalitis viruses and HIV as well as Staphylococcus aureus staph infections. Laboratory facilities Back to top Agents that require enhanced precautions BSL-2. BSL2 Laboratory Closeout Checklist.

Eating drinking smoking handling contact lenses and applying cosmetics are not permitted in the work areas. The purpose of containment is to reduce or eliminate exposure to people or the environment to potentially hazardous agents. Standard microbiological practices C.

Sign and date the completed checklist. BMBLC5 Eye face and respiratory protection should be used in rooms containing infected animals as determined by the risk assessment. BSL-2 is suitable for work with agents associated with human disease and pose moderate hazards to personnel and the environment.

Persons wash their hands after they handle viable materials and animals after removing gloves and before leaving the laboratory. 112021 If a lab has windows that open they are fitted with fly screens or the lab has a policy not to open windows. Are animals and plants not associated with the work prohibited from the laboratory.

BSL2 This biosafety level covers laboratories that work with agents associated with human diseases ie. LABORATORY BIOSAFETY LEVEL 2 CRITERIA Biosafety in Microbiological and Biomedical Laboratories BMBL 6th Edition Section IV. Personnel are trained to handle pathogenic agents and are supervised by those with advanced trainingundefined Biosafety cabinets or other physical containment for all procedures that can generate infectious aerosols or splashes.

Personnel with medical conditions that may make them more susceptible to infection ie. BMBLC4-c Hand washing protocols must be rigorously followed. Is medical follow-up obtained after spills accidents and potential exposures.

This protocol provides information for both biosafety level 1 BSL-1 and biosafety level 2 BSL-2. CDC Import Permit Inspection Checklist for ABSL-2 Laboratories BMBL 6 th Edition Insp. All personnel wash their hands after they handle viable materials and animals after removing gloves and before leaving the laboratory.

Mechanical pipetting devices are available and used for all pipetting. It differs from BSL-1 in that. Microsoft Word - BSL-2 Checklistdoc Version.

Biosafety Level 2 Checklist 2 Rev. Lab personnel receive annual updates or additional training when procedural or policy changes occur. BSL-2 Inspection Checklist - Rev.

This includes BSL1 or BSL2 materials stored in any lab spaces refrigerators freezers incubators and cold rooms. Mouth pipetting is prohibited and only mechanical pipetting devices are used. Cultures of microorganisms cell lines blood fresh tissue sharps carcasses etc must be removed prior to vacating the laboratory spaces.


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